Stephan Tirier

Stephan Tirier, as the Director of Assay Development at Resolve BioSciences, plays a pivotal role in advancing the company's cutting-edge spatial multi-omics technologies. His leadership focuses on the development and optimization of the Molecular Cartography workflow, a transformative approach that enables high-resolution, multiplexed imaging of gene expression within intact biological tissues. Under his guidance, the Assay Development team is responsible for designing highly specific panels of probes, refining sample preparation protocols, and innovating detection chemistries that maximize sensitivity and spatial resolution. Stephan’s expertise in single-cell genomics, RNA-Seq, and next-generation sequencing (NGS) is instrumental in ensuring the robustness and scalability of these assays, facilitating precise single-cell analysis within complex tissue microenvironments.

A key aspect of his role involves integrating the highly multiplexed smRNA-FISH assay with complementary modalities such as Cite-Seq, enabling comprehensive multi-omic profiling at the single-cell level. His deep understanding of cancer biology and cell culture techniques informs the development of assays tailored for tumor microenvironment studies, immune profiling, and biomarker discovery. Stephan’s proficiency in bioinformatics tools like KNIME and light microscopy techniques further enhances the team's ability to analyze and interpret spatial transcriptomics data, driving insights into cellular heterogeneity and tissue architecture.

Through his strategic leadership, Resolve BioSciences is pushing the boundaries of spatial biology, providing researchers with powerful tools to unravel the complexities of biological systems. Stephan’s commitment to innovation and his multidisciplinary expertise position him as a key driver in transforming spatial multi-omics from a research frontier into a practical, widely accessible technology for biomedical discovery.