Vera Rapoport's Email Addresses & Phone Numbers

Technically sophisticated, well-organized and dedicated Biologist, equipped with hands-on experience in molecular techniques and in CE-IVD assay design and development. Expert at managing multiple projects by setting priorities and rendering strategic direction to ensure accomplishment of simple to complex tasks within specific deadlines. Highly capable of data interpretation and troubleshooting. Excellent team player with strong work ethic,...

Technically sophisticated, well-organized and dedicated Biologist, equipped with hands-on experience in molecular techniques and in CE-IVD assay design and development. Expert at managing multiple projects by setting priorities and rendering strategic direction to ensure accomplishment of simple to complex tasks within specific deadlines. Highly capable of data interpretation and troubleshooting. Excellent team player with strong work ethic, well-defined problem-solving and decision-making abilities.Sr Research Associate @ Performing R&D projects through all stages of development: planning, sequence files, documentation, design, testing, optimization, validation and final product release. Developing Multiplex Real Time PCR and RT-qPCR assays for fusion genes detection, SNPs and genotyping analyses. Processing functional assay validation studies such as sensitivity, specificity, cross-reactivity, reproducibility and reliability, as well as optimization of the assay. Developing QC standards, High Positives and Low Positives for QA procedures and manufacture. Analyzing statistical data received from the developed assays and adapting/optimizing the assays to achieve technical objectives. Performing key validation experiments required for troubleshooting final product QA. Releasing batches based on QC data and production data such as statistical analyses. Processing clinical specimens used in experiments and studies for the assays evaluation. Presenting research findings and experimental results at the group meetings. Managing product QA through final product release to the customers. Drafting and revising SOPs, laboratory protocols and final production recipes. Maintaining accurate and cGMP compliant documentation of experiments. Controlling production batch preparation and QC procedures in compliance with cGMP. From May 2013 to Present (2 years 8 months) Molecular Research Associate @ Performed Oligonucleotide primer design, gene-specific assays set-up, data collection and analysis of the raw data, using LinRegPCR software (calculation of the relative expression ratio based on efficiency calibrated Pfaffle model). RNA isolation, RT-PCR and cDNA libraries construction. Vector design and Cloning cDNA fragments into plasmids using techniques of Blunt-end cloning and A‘overhangs sticky-ends cloning; vectors construction by restriction digests, DNA ligation, bacterial transformation, colony PCR, plasmid minipreps and agarose gel electrophoresis for the cloned product confirmation. Sample prep for NGS and post-sequencing analyses. Detection of the untranslated ends of the gene using 5’RACE and 3’RACE techniques. Promoter-reporter gene fusions and injections of the constructs for in vivo gene and protein expression analyses. Additional Skills: Good working knowledge of cell and tissue culture techniques, such as mammalian and plant cells culture in aseptic cell culture techniques, media preparation and observation of the viable cells, subculture of monolayer cells, in vitro bioassays like cell proliferation, adhesion, migration, apoptosis; cryopreservation; Flow Cytometry. Excellent lab experience in biochemistry techniques: detection of proteins by ELISA, detection of nucleic acids using FISH, detection of cells subpopulation by specific antibody binding using FACS, polyacrylamide gels electrophoresis; Flow Cytometry; Column chromatography such as HPLC, Affinity, Ion Exchange and gel filtration. From September 2009 to May 2013 (3 years 9 months) Master of Science, Molecular Biology @ California State University-Northridge From 2011 to 2013 Bachelor of Science, Biotechnology Engineering @ ORT Braude College From 2005 to 2009 Vera Rapoport is skilled in: PCR, Biotechnology, Cell Culture, Molecular Biology, Western Blotting, Biochemistry, ELISA, Molecular Cloning, Molecular Genetics, Tissue Culture, Cell Biology, Fluorescence Microscopy, Immunohistochemistry, Protein Chemistry, SDS-PAGE