I am a Pharmacologist and research scientist with experience of drug evaluation and development. # Excellent communication & Leadership Skill # Expert in managing clinical and preclinical trials, # Expert in behavioral and molecular pharmacology # Interested in challenging roles in product oriented industry focusing on developing/testing/marketing lead molecules, herbal drug and pharmaceutical preparations. # In-depth understanding of pharmacodynamic and pharmacokinetic studies, IRB and FDA guidelines, drug development process (phase I to IV) Specialties : ELISA Assay development, HPLC, GC-MS, ViiA™ 7 Real-Time PCR System, Multiplex assay using Luminex, Flow cytometry, Cell culture, Animal microsurgery, Animal Behavioral Experiment, Cell Imaging, Transmission Election Microscope, Confocal Microscopy, Cytokines, Catecholamines, Transfection, siRNA. Current Area of Research : Mitochondria dysfunction is the main cause of several diseases. My area of interest is to study the mitochondrial stress in metabolic imbalance such as burn trauma, obesity, diabetes, aging etc. We measured beta adrenergic receptors, mitochondrial morphology, mitochondrial function, metabolic hormones, proteins, genes and histological changes in adipose tissue of burn patients. My long term goal is to identify the lead molecule for novel drug target site and develop its pharmaceutical preparation.

Research Scientist @ Burn injury is characterized a by hypermetabolic response and severe adrenergic stress. Despite advances in burn care, hypermetabolism remains a significant clinical problem for burned children. Chronic adrenergic stress alters tissue architecture and causes lipolysis, cardiac stress and thermogenesis, however the exact mechanism(s) governing these responses are not known.We are studying the impact of burn injury of adipose tissue tranformation, thermogesis and inflammatory cytokines, hormones, mitochondrial function etc. Technical Specialties : - Clinical trial management, conducting hyperinsulinemic-euglycemic clamps clinical studies in burn patients, human sample extraction, - Data management and analysis, project report and manuscript preparation - Laboratory analysis (Multiplex magnetic beat assay of hormones, cytokines; ELISA of Irisin, adiponectin, BMP, Insulin, Catecholamine; Duplex Real Time PCR of genes, Immunohistochemistry and immunofluorescence of UCP1, macrophages, ADR Beta etc - Electron microscopy of adipose tissue and muscle. - Histology of adipose tissue and muscle From November 2011 to Present (4 years 2 months) Post Doc Fellow @ During my post doc training, we reported that ENPP1 (a negative modulator of insulin receptor and causing insulin resistance) over-expression in adipocytes induces fatty liver, hyperlipidemia and dysglycemia, thus recapitulating key manifestations of the metabolic syndrome (AJP-Endocrinology and Metabolism. 2011). We reported the role of ER stress in expression of ATF3 and collagen 6a3 in 3T3 cells (FASEB Journal, 2012). In a clinical study, the oral administration of combination of Lycopene and Isoflavones showed moderate improvement on glucose and lipid metabolism in human subject and demonstrated as a safe and effective non-pharmacological alternative treatment to improve insulin resistance and metabolic syndrome. (The Journal of Functional Foods in Health and Disease 2010). We also studied effect of R-equol (metabolite of phytoestrogen daidzein) on non-genomic estrogenic signaling mechanism of endogenous estrogen estradiol and pharmaceutical estrogen ethynil estradiol in pituitary tumor cells (FASEB Journal 2013). Technical Specialties : - Semi-quantitative plate assay, nongenomic signaling pathway analysis (P38, MAPK, JNK), calcium signaling, pituitary hormone, Cell culture, writing manuscript, maintain cell line etc - Adipose tissue (abdominal and gluteal fat tissue) biopsy collection, Adipose tissue histology, and Cell size measurement, hyperinsulinemic-euglycemic clamps, Cell pellet from blood, genotyping etc, Cell culture. - Collection, processing and storage of saliva, collection of adipose tissue biopsy, adipose tissue histology and cell size measurement. - ENPP1 over-expressed transgenic mice breeding, genotyping, high fat diet pair feeding, intraperitoneal glucose tolerance test, insulin challenge test, blood and organ collection, gene expression profiling in adipose tissue and liver, adipose tissue histology and cell size measurement, adipose tissue isolation, RT-PCR, Genotyping From July 2009 to October 2011 (2 years 4 months) Post Doc Fellow @ Behavioral and Neurochemical Studies of mechanism of drug addiction and food compulsive disorders. From October 2008 to March 2009 (6 months) Assistant Professor @ Higher education to post graduate and phd student and supervised them for research activity. From January 2008 to September 2008 (9 months) Research Associate @ DST Project : Assessment of marrow derived stem cell implantation in experimentally induced brain stroke mouse model” Supervisor : Prof. S. Prabhakar, Head, Department of Neurology, Brain Stroke : Effect of marrow derived stem cell implantation Neurodegenerative disease represents a large group of neurological disorders such as stroke and Alzhiemer’s disease, affecting specific subsets of neurons. In this study we plan to evaluate the therapeutic potential of neurogenesis induced by stem cell implantation in an experimentally induced mouse model. We will induce global neurodegeneration by hypoxia and selective neurodegeneration by MCAO and electro-burning method followed by assessment by neurobehavioral test and infarct size measurement, We plan to investigate the expression of various growth factors, adhesion molecules and chemokine. From September 2007 to February 2008 (6 months) PhD Scholar @ We investigated the modification of intracellular signaling of long term potentiation by a series of amnesic agents and reported the contribution of protein kinase-CREB pathway for an antiamnesic effect of Bacopa monniera. We also noticed first time possible mechanism of retrograde amnesia induced by triazolodiazepine around PAF receptor. They also investigated the neuroprotective effect of Bacopa monneira on ischemia induced brain injury in ICA (internal carotid artery occlusion) rat model and proposed an involvement of signally pathway of oxidative stress. From 2003 to 2008 (5 years) Senior Research Fellow @ ICMR Project : Neuroprotective effect of Bacopa monniera on Ischemia induced reperfusion induced brain injury. Cognition impairment is originated due to brain injury in brain stroke. We found Bacopa’s moderate neuroprotective effect on ischemia induced brain stroke developed by internal carotid artery occlusion method in rat model. This study would help in the assessment of the therapeutic capacity of Bacopa monniera in the reversal of brain stroke and the associated amnesia. Bacopa’s antioxidant antiamnesic and antiapoptotic properties are well documented. In order to delineate the neuroprotective mechanism, we focused on oxidative pathway by measuring SOD, Catalase, LPox, Nitrite, Nitrate, GPx etc. From November 2005 to November 2007 (2 years 1 month) Junior Research Fellow @ ICMR Project : The contribution of prothrombotic state in etiology of ischemic stroke in young Indian population Supervisor : Prof. S. Prabhakar, Head, Department of Neurology, Duration : 3 years Research Findings : Hyperhomocysteinemia has been proposed world wide as an important risk factor for ischemic stroke. A procoagulant work up including Protein C, Protein S, antithrombin III deficiency. APLA etc were performed in patient. Fasting serum homocysteine levels were measured at least three months after the acute ischemic episode. Concentration of total homocysteine levels (tHcy) were found to be significantly high in patients with ischaemic stroke as compared to controls (9.91 umol/L vs. 8.00 umol/L). The levels were significantly high in both age groups of younger and older patients and in either sex group as compared to controls. A strong positive correlation was also observed between hypertension, smoking and high tHcy levels in the present study. From August 2002 to August 2005 (3 years 1 month) Chandigarh Area, IndiaLecturer @ Teaching Pharmacology, Human anatomy and physiology From November 2001 to August 2002 (10 months) Lecturer @ Teaching pharmacy, pharmacology etc From July 1997 to May 1998 (11 months)

PhD, Neuroscience @ Postgraduate Institute of Medical Education and Research From 2002 to 2008 Industrial Diploma in Drug Designing and Patenting (IDDP), Drug Design and Patenting @ Bioinformatics Institute of India, Noida From 2006 to 2007 M. Pharma., Pharmacology @ Punjabi University From 1998 to 2002 B. Pharma, Pharmacy @ Doctor Harisingh Gour Vishwavidyalaya From 1994 to 1997 Advance Diploma in Applied Bioinformatics (ADAB), Applied Bioinformatics @ Bioinformatics Institute of India Noida Manish Saraf is skilled in: Neuroscience, Immunohistochemistry, Pharmacology, Molecular Biology, Biochemistry, Clinical Research, ELISA, Western Blotting, Animal Models, Assay Development, HPLC, Physiology, Drug Discovery, Cell Culture, Bioinformatics