Daniel PhD's Email

Highly motivated biochemical engineer with demonstrated publication record and leadership experience. Skills include: Mammalian Cell Culture: Experience with the culture of several cell lines including human (H1, H9) and mouse (E14, R1) embryonic stem cells (ESCs), HEK-293 and MIN-6; isolation of DNA, RNA, and protein; real time PCR; gel electrophoresis, flow cytometry, immunofluorescence imaging, western blot analysis, karyotype analysis, confocal microscopy, and scanning electron microscopy (SEM). Small animal surgery and handling. Bacterial Cell Culture: Creation of competent bacterial cells, their transformation, selection, and expansion, and the extraction and purification of plasmids. Stirred Tank Bioreactor: Adaptation and culture of several cell types including ESCs and insulin-producing cells and use of microcarriers. Characterization and optimization of culture process variables. Computer Programs and Process Simulators: FORTRAN 77, Visual Basic, Aspen, Hysys, Clone Manager, Adobe Photoshop, Excel, Word. Leadership: Supervised five undergraduate students and assisted them with learning new techniques. Served as Graduate Student Association President, an elected position. Directly supervised three staff, led meetings, introduced speakers, conducted presentations, and represented over 6000 graduate students at the University.

Research Scientist III-Cell Biologist @ -Provide stem cell culture expertise to the Upstream Processing team in the Bioprocess Division. -Coordinate and participate in product development activities. -Develop new technologies to support cell growth. From June 2010 to Present (5 years 7 months) Teaching Asst./Research Asst./GSA President @ Graduate Student Association President, University at Buffalo, Amherst, NY 6/08-5/09 Elected to paid leadership position. Directly supervised three staff, led meetings, introduced speakers, interacted with deans, faculty, and students from a range of disciplines, and represented over 6000 graduate students at the University. Research Assistant, University at Buffalo, Amherst, NY 9/06-5/08 Oversaw research projects. Supervised up to five undergraduate students and taught research techniques. Teaching Assistant, University at Buffalo, Amherst, NY 8/03-12/05 Chemical Plant Design. Assisted with computer lectures for hysys, graded homework and exams, held office hours, and assisted students with term projects. Materials Science & Corrosion Lab. Taught laboratory class, graded assignments and exams, and held office hours for lecture section of class. From August 2003 to May 2009 (5 years 10 months) Carpenter @ Built and remodeled residential homes using several types of hand tools and power tools. Worked on all aspects of building including reading blueprints, installing foundations, framing, sheathing, roofing, plumbing, electrical work, trimming, and finish work. From October 1998 to June 2003 (4 years 9 months) Programming Engineer @ Designed and coded software programs to size and priced heat exchangers, and separators. From June 1997 to December 1997 (7 months)

Ph.D., Chemical and Biological Engineering @ University at Buffalo From 2003 to 2009 B.S., Chemical Engineering @ University at Buffalo From 1994 to 1998 JJHS